CA2371011A1 - Polyionic coatings in analytic and sensor devices - Google Patents

Polyionic coatings in analytic and sensor devices Download PDF

Info

Publication number
CA2371011A1
CA2371011A1 CA002371011A CA2371011A CA2371011A1 CA 2371011 A1 CA2371011 A1 CA 2371011A1 CA 002371011 A CA002371011 A CA 002371011A CA 2371011 A CA2371011 A CA 2371011A CA 2371011 A1 CA2371011 A1 CA 2371011A1
Authority
CA
Canada
Prior art keywords
poly
group
substrate
functionalized
copolymer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002371011A
Other languages
French (fr)
Other versions
CA2371011C (en
Inventor
Marcus Textor
Donald L. Elbert
Stephanie Finken
Laurence Ruiz-Taylor
Nicholas D. Spencer
Rolf Hofer
Jeffrey A. Hubbell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eidgenoessische Technische Hochschule Zurich ETHZ
Universitaet Zuerich
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2371011A1 publication Critical patent/CA2371011A1/en
Application granted granted Critical
Publication of CA2371011C publication Critical patent/CA2371011C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/14546—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring analytes not otherwise provided for, e.g. ions, cytochromes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
    • A61B5/1459—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using optical sensors, e.g. spectral photometrical oximeters invasive, e.g. introduced into the body by a catheter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/10—Macromolecular materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/819—Multifunctional antigen or antibody
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se

Abstract

Multifunctional, polyionic copolymers are synthesized on/or applied to substrate surfaces for analytical and sensing purposes, the coatings being particularly useful for suppression of non-specific interaction, adsorption or attachment. Groups can be coupled to, integrated into or absorbed to the multifunctional polymer that are able to recognize, interact with and bind specifically to target analyte molecules. These materials can also be used to modulate biological interactions upon substrate surfaces for use as selective implant surfaces that resist cell attachment and may optionally promote the attachment of specific cell types or induce a particular cellular behaviour.
The multifunctional polymer coatings typically include brush copolymers based on a polycationic or polyanionic backbone with side chains that control interaction with the environment, such as poly(ethylene glycol) or poly(ethylene oxide)-based side chains that decrease cellular adhesion, and analyte-specific side chains.

Claims (43)

1. A method for reducing non-specific adsorption comprising applying to or coating onto a surface or substrate in an analytical or biosensing device a polyionic multifunctional copolymer, wherein the polyionic multifuntional copolymer comprises non-interactive polymer side chains grafted onto a charged polyionic polymeric backbone, and wherein the substrate or surface comprises a material selected from the group consisting of metals and metal oxides.
2. The method of claim 1 where the polymeric backbone has a cationic charge at a pH greater than 4.
3. The method of claim 2 wherein the polymeric backbone comprises a cationic backbone selected from the group consisting of polymers comprising amino acids containing side groups imparting a positive charge to the backbone at pH greater than 4, polysaccharides, polyamines, polymers of quarternary amines, and charged synthetic polymers.
4. The method of claim 3 where the cationic backbone comprises one or more units selected from the group consisting of lysine, histidine, arginine and ornithine in a D-, L-, or DL configuration, chitosan, partially deacetylated chitin, amine-containing derivatives of neutral polysaccharides;
poly(aminostyrene), poly(aminoacrylate), poly (N-methyl aminoacrylate), poly (N-ethylaminoacrylate), poly(N,N-dimethyl aminoacrylate), poly(N,N-diethylaminoacrylate), poly(aminomethacrytate), poly(N-methyl, amino-methacrylate), poly(N-ethyl aminomethacrylate), poly(N,N-dimethyl aminomethacrylate), poly(N,N-diethyl aminomethacrylate), poly(ethyleneimine), poly(N,N,N-trimethylaminoacrylate chloride), poly(methyacrylamidopropyltrimethyl ammonium chloride), polyethyleneimine, polyamino(meth)acrylate, polyaminostyrene, polyaminoethylene, poly(aminoethyl)ethylene, polyaminoethylstyrene, and N-alkyl derivatives thereof.
5. The method of claim 1 wherein the polymeric backbone has an anionic charge at a pH greater than 4.
6. The method of claim 5 wherein the anionic backbone comprises a polymer selected from the group consisting of polymers comprising amino acids containing pendant charged groups imparting a negative charge to the backbone at pH greater than 4, polysaccharides, and charged synthetic polymers with pendent negatively charged groups.
7. The method of claim 6 wherein the anionic backbone comprises one or more units selected from the group consisting of polyaspartic acid, polyglutamic acid, alginate, carrageenan, furcellaran, pectin, xanthan, hyaluronic acid, heparin, heparan sulfate, chondrointin sulfate, dermatan sulfate, dextran sulfate, poly(meth)acrylic acid, oxidized cellulose, carboxymethyl cellulose and crosmarmelose, maleic acid polymers, and fumaric acid polymers.
8. The method of claim 1 wherein the non-interactive polymer is selected from the group consisting of poly(alkylene glycols), poly(alkylene oxides), neutral water-soluble polysaccharides, polyvinyl alcohol, poly-N-vinyl pyrrolidone, phosphoryl choline derivatives, non-cationic poly(meth)acrylates and combinations thereof.
9. The method of claim 1 where the non-interactive polymers are-fully or partially modified at or near the terminal end of the polymers with reactive groups suitable for further functionalization.
10. The method of claim 9 wherein the reactive groups are selected from the group consisting of hydroxy (-OH), carboxy (-COOH], ester (-COOR), thiol (-SH), N-hydroxy-succinimidyl, maleimidyl, quinone, vinylsulfone groups, and combinations thereof.
11. The method of claim 1 where some or all of the non-interactive polymers are partially or fully functionalized at or near the free terminal position of the non-interactive polymer with a functional molecule which will specifically recognize and interact with target molecules.
12. The method of claim 11 wherein the functional molecule is selected from the group consisting of protein ligands, polynucleotides, carbohydrate or sugar ligands, simple organic molecules, and combinations thereof.
13. The method of claim 12 wherein the functional molecule is biotin.
14, The method of claim 1 wherein the material has a negative charge and is selected from the group consisting of oxides with isoelectric points below 9 and materials having a negative charge introduced by a surface pretreatment.
15. The method of claim 14 wherein the material is selected from the group consisting of tantalum, niobium, titanium, hafnium, silicon, iron and chromium oxides; iron, chromium, steel, tantalum, niobium, titanium, hafnium, gold, and silver which either carry a negative charge at the pH used due to the presence of native oxide films or which have been treated to induce a negative charge to the material.
16. The method of claim 1 wherein the material has a positive charge and is selected from the group consisting of oxides that have an isoelelectric point (IEP) above 5, and oxides or metals that have been treated to impose a positive charge onto the surface at the pH of greater than 4.
17. The method of claim 16 wherein the material is selected from the group consisting of tantalum, niobium, titanium, hafnium, silicon, iron and chromium oxides; iron, chromium, steel, tantalum, niobium, titanium, hafnium, gold, and silver which either carry a positive charge at the pH used due to the presence of native oxide films or which have been treated to induce a positive charge to the material.
18. The method of claim 11 comprising exposing the functionalized copolymers to analyte which is bound by the functional group in a fluid phase followed by adsorption of the functionalized copolymer entity onto a substrate or surface.
19. The method of claim 11 comprising exposing the functionalized copolymers to analyte which is bound by the functional group, wherein the functionalized copolymers are adsorbed unto a substrate or surface before or after binding the analyte.
20. The method of claim 11 comprising applying non-interactive polyionic multifunctional copolymers to a surface or substrate in, on, or forming an analytical or sensing device having a charge on the surface or substrate opposite to the charge of the copolymer, wherein some of the non-interactive polymer side chains are functionalized with a functional moiety which will specifically recognize and interact with target molecules and some of the non-interactive polymer side chains are not functionalized.
21. The method of claim 20 wherein the functionalized and non-functionalized copolymers are applied sequentially.
22. The method of claim 21 wherein the functionalized copolymers are adsorbed to the substrate or surface and exposed to analyte, then non-functionalized copolymers are adsorbed to the substrate or surface.
23. The method of claim 11 wherein the functionalized copolymer is attached to the substrate or surface by physisorption or chemisorption.
24. The method of claim 11 wherein the functionalized copolymer is attached to the substrate or surface by covalent, chemical binding.
25. The method of claim 11 wherein the surface is patterned with areas with adsorbed, non-modified and/or, non-functionalized polyionic copolymer and areas with adsorbed modified and/or functionalized polyionic copolymer.
26. The method of claim 25 wherein the patterns have dimensions parallel to the surface in the millimeter, micrometer or submicrometer range.
27. The method of claim 25 wherein the patterning is done by fluidic, microfluidic, stamping or microcontact printing.
28. The method of claim 25 wherein the polyionic copolymer is applied to the substrate or surface, then functional molecule is bound to the copolymer in a defined surface pattern by fluidic, microfluidic, stamping, microcontact printing or ink jet techniques.
29. The polymeric coated substrate or surface formed by the method of any of claims 1-28.
30. The polymeric coated substrate or surface formed by the method of any of claims 1-28 applied to a surface or substrate in a device or material for use in analytical or biosensing devices.
31. The use of the polymeric coated substrate or surface of claim 29 for qualitatively or quantitatively measuring or detecting analyte.
32. The use of claim 31 wherein the analyte is selected from the group consisting of polynucleotides, peptides, proteins, carbohydrates, sugars, and glycoproteins.
33. The use of claim 31 wherein the amount of analyte immobilized at the polyionic copolymer coated substrate or surface is determined by measuring or using electromagnetic radiation or using a method like absorption, scattering, emission, thermal phenomena or combinations thereof.
34. The use of claim 33 wherein the amount of analyte is determined by using waveguides.
35. The use of claim 34 wherein the waveguides comprise one or more optical coupling elements for incoupling excitation light to the waveguides.
36. The use of claim 35 wherein the optical coupling elements are selected from the group consisting of prism couplers, evanescent couplers comprising joined optical waveguides with overlapping evanescent fields, end face couplers with focusing lens, preferably cylindrical lens located adjacent to the waveguide and gating couplers and combinations thereof.
37. The use of claim 36 wherein the optical coupling elements are diffractive gratings selected from the group consisting of relief gratings with any profile such as rectangular, triangular or sinusoidal profile, and phase gratings or volume gratings with a periodical modulation of the refractive index in the waveguide and combinations thereof.
35. The use of claim 37 wherein one or more of the optical coupling elements are diffractive relief gratings with a rectangular profile.
39. The method of claim 33 wherein analyte is detected using a continuous type of process.
40. The use of claim 33 wherein the functionalized polyionic copolymer is applied in a pattern, and detection is localized to specific areas of the pattern.
41. The use of claim 40 wherein the pattern includes regions of different functional molecules for detection of multiple analytes.
42. The use of the polymeric coated substrate or surface of claim 29 to modify cell to surface interactions on a metal or metal oxide surface.
43. The use of claim 42 wherein the substrate a material for analytical or biosensing devices.
CA002371011A 1999-04-28 2000-04-28 Polyionic coatings in analytic and sensor devices Expired - Lifetime CA2371011C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US13139199P 1999-04-28 1999-04-28
US13140299P 1999-04-28 1999-04-28
US60/131,402 1999-04-28
US60/131,391 1999-04-28
US18461600P 2000-02-24 2000-02-24
US60/184,616 2000-02-24
PCT/US2000/011708 WO2000065352A1 (en) 1999-04-28 2000-04-28 Polyionic coatings in analytic and sensor devices

Publications (2)

Publication Number Publication Date
CA2371011A1 true CA2371011A1 (en) 2000-11-02
CA2371011C CA2371011C (en) 2009-12-22

Family

ID=27384152

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002371011A Expired - Lifetime CA2371011C (en) 1999-04-28 2000-04-28 Polyionic coatings in analytic and sensor devices

Country Status (8)

Country Link
US (1) US6884628B2 (en)
EP (1) EP1190252B1 (en)
JP (1) JP4647792B2 (en)
AT (1) ATE419528T1 (en)
AU (1) AU769571B2 (en)
CA (1) CA2371011C (en)
DE (1) DE60041255D1 (en)
WO (1) WO2000065352A1 (en)

Families Citing this family (241)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6692914B1 (en) 1999-07-02 2004-02-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
US20030059809A1 (en) * 1999-12-17 2003-03-27 Todor Vujasinovic Biochips,preparation and uses
US7163712B2 (en) * 2000-03-03 2007-01-16 Duke University Microstamping activated polymer surfaces
US6406777B1 (en) * 2000-06-14 2002-06-18 The United States Of America As Represented By The Secretary Of The Navy Metal and glass structure for use in surface enhanced Raman spectroscopy and method for fabricating same
DE10036907B4 (en) * 2000-07-28 2012-03-22 Xantec Bioanalytics Gmbh Process for the preparation of a coating on a gold vapor-deposited glass substrate, coating produced by this process and their use
US7678539B2 (en) * 2000-08-10 2010-03-16 Corning Incorporated Arrays of biological membranes and methods and use thereof
EP1316599B1 (en) * 2000-08-21 2014-03-26 National Institute of Advanced Industrial Science and Technology Magnetic particles and process for producing the same
WO2002056021A2 (en) * 2001-01-10 2002-07-18 Symyx Technologies Inc Polymer brushes for immobilizing molecules to a surface
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
JP4672966B2 (en) * 2001-03-09 2011-04-20 アポロ バイオテクノロジー，インコーポレイテッド Conjugate probes and optical detection of analytes
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US6844028B2 (en) 2001-06-26 2005-01-18 Accelr8 Technology Corporation Functional surface coating
US7501157B2 (en) * 2001-06-26 2009-03-10 Accelr8 Technology Corporation Hydroxyl functional surface coating
US6989267B2 (en) 2001-07-02 2006-01-24 Agilent Technologies, Inc. Methods of making microarrays with substrate surfaces having covalently bound polyelectrolyte films
EP1411816A4 (en) * 2001-07-09 2005-09-28 Univ Arizona State Affinity biosensor for monitoring of biological process
AU2002338007A1 (en) * 2001-08-27 2003-03-18 Zeptosens Ag Surface for the immobilisation of nucleic acids
US20040253596A1 (en) * 2001-08-27 2004-12-16 Michael Pawlak Bioanalytical recognition surface with optimized recognition element density
US9671396B2 (en) * 2001-09-05 2017-06-06 Joon Won Park Solid substrate comprising array of dendrons and methods for using the same
EP1425583A1 (en) * 2001-09-12 2004-06-09 Eidgenössische Technische Hochschule Zürich Device with chemical surface patterns
EP1435520A4 (en) * 2001-09-21 2005-02-02 Takara Bio Inc Support for ligand immobilization
FI118061B (en) 2001-09-24 2007-06-15 Beanor Oy Procedure and bio donor for analysis
JP2005513479A (en) * 2001-12-21 2005-05-12 センス プロテオミック リミテッド Probe for mass spectrometry
US8043868B2 (en) 2001-12-21 2011-10-25 Imec Method and apparatus for detecting an analyte
EP1321761B1 (en) * 2001-12-21 2006-08-23 Interuniversitair Micro-Elektronica Centrum Method of detecting an analyte
FI115166B (en) * 2001-12-31 2005-03-15 Biofons Oy Diagnostic procedures
US7635463B2 (en) 2002-02-27 2009-12-22 Pharmain Corporation Compositions for delivery of therapeutics and other materials
ATE494010T1 (en) * 2002-02-27 2011-01-15 Pharmain Corp THERAPEUTIC DELIVERY COMPOSITIONS AND OTHER MATERIALS AND METHODS FOR THE PRODUCTION AND USE THEREOF
US20050260259A1 (en) * 2004-04-23 2005-11-24 Bolotin Elijah M Compositions for treatment with glucagon-like peptide, and methods of making and using the same
AU2002233097A1 (en) * 2002-02-28 2003-09-09 Eidgenossische Technische Hochschule Zurich Oberflachentechnik Biomedical implant surfaces with osteogenic properties
JP2005527807A (en) * 2002-04-12 2005-09-15 ミクロナス　ゲーエムベーハー Method for immobilization of molecules on surfaces
JP2005523372A (en) * 2002-04-16 2005-08-04 エイドゲントシッシュ テクニーシェ ホッシュール チューリッヒ Environmentally compatible additives for aqueous lubricants
US20030198967A1 (en) * 2002-04-23 2003-10-23 Matson Robert S. Multi-functional microarrays and methods
US7010182B2 (en) * 2002-07-31 2006-03-07 Luna Innovations Incorporated Biosensors having enhanced environmental sensitivity
US7998699B2 (en) 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
US7384742B2 (en) * 2002-08-16 2008-06-10 Decision Biomarkers, Inc. Substrates for isolating reacting and microscopically analyzing materials
US20090118605A1 (en) * 2002-08-30 2009-05-07 Northwestern University Surface-enhanced raman nanobiosensor
US20040043508A1 (en) * 2002-09-03 2004-03-04 Frutos Anthony G. Polymer-coated substrates for binding biological molecules
US20040062882A1 (en) * 2002-09-30 2004-04-01 Andrea Liebmann-Vinson Cell adhesion resisting surfaces
ES2208121B1 (en) * 2002-11-29 2005-08-16 Consejo Sup. Investig. Cientificas ANTIBODIES AND IMMOBILIZED ANTIGENS ON SILICON MAGNETIC PARTICLES AS BIOSENSORS.
US7070922B2 (en) * 2002-12-04 2006-07-04 International Business Machines Corporation Surface treatment
US20040121339A1 (en) * 2002-12-19 2004-06-24 Jizhong Zhou Special film-coated substrate for bio-microarray fabrication and use thereof
CN100347545C (en) * 2003-04-08 2007-11-07 成都夸常科技有限公司 Method of preoceeding qualitative and/or quantitative analysis against target substance in sample and its detecting device
JP4006523B2 (en) * 2003-04-10 2007-11-14 独立行政法人産業技術総合研究所 Protein array and production method thereof
AU2004230592B2 (en) * 2003-04-14 2007-10-04 Caliper Life Sciences, Inc. Reduction of migration shift assay interference
DE10320312A1 (en) * 2003-05-06 2004-12-02 Friz Biochem Gmbh Substrate as a carrier for ligates
US20040228766A1 (en) * 2003-05-14 2004-11-18 Witty Thomas R. Point of care diagnostic platform
US7160867B2 (en) * 2003-05-16 2007-01-09 Isotechnika, Inc. Rapamycin carbohydrate derivatives
US7731906B2 (en) 2003-07-31 2010-06-08 Handylab, Inc. Processing particle-containing samples
US9206414B2 (en) * 2003-08-13 2015-12-08 Axcelon Biopolymers Corporation Anisotropic nanocomposite hydrogel
ATE430812T1 (en) 2003-08-15 2009-05-15 Univ South Florida MATERIALS AND METHODS FOR CAPTURE PATHOGENS AND SEPARATE AURINTRICARBOXYLIC ACID FROM A SAMPLE
US20050070027A1 (en) * 2003-09-30 2005-03-31 Jacques Gollier Double resonance interrogation of grating-coupled waveguides
JP2006514299A (en) * 2003-10-27 2006-04-27 センス プロテオミック リミテッド Enzyme arrays and assays
US20050244898A1 (en) * 2003-11-19 2005-11-03 Cohen Robert E Large-area two-dimensional non-adhesive cell arrays for sensing and cell-sorting applications
US7510942B2 (en) * 2003-11-25 2009-03-31 Arizona Board Of Regents, Acting For And On Behalf Of Arizona State University Molecular modifications of metal/dielectric interfaces
US20050131513A1 (en) * 2003-12-16 2005-06-16 Cook Incorporated Stent catheter with a permanently affixed conductor
US20050180957A1 (en) * 2004-01-16 2005-08-18 Scharp David W. Method of using fibrin-bound angiogenic factors to stimulate vascularization of transplant site of encapsulated cells
US7723126B2 (en) * 2004-03-24 2010-05-25 Wisconsin Alumni Research Foundation Plasma-enhanced functionalization of inorganic oxide surfaces
JP4535490B2 (en) * 2004-04-09 2010-09-01 株式会社資生堂 Protein adsorption prevention method
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
AU2005241080B2 (en) 2004-05-03 2011-08-11 Handylab, Inc. Processing polynucleotide-containing samples
WO2005114194A1 (en) * 2004-05-21 2005-12-01 Mds Inc. Doing Business Through Its Mds Pharma Services Division Method of quantifying the cell-binding properties of a medical device
EP1771214A2 (en) * 2004-06-16 2007-04-11 Affinergy Inc. Biofunctional coatings
US10150945B2 (en) * 2004-07-23 2018-12-11 Jnc Corporation Cell culture device and manufacturing method therof
US20060029961A1 (en) * 2004-08-04 2006-02-09 Goh M C Patterned surfaces and their use in diffraction-based sensing
US7595157B2 (en) * 2004-08-19 2009-09-29 Biocept, Inc. Microarrays utilizing hydrogels
US20110177955A1 (en) * 2004-10-12 2011-07-21 Luis Alberto Burzio Multiplexed protein adsorption assay
US20060110594A1 (en) * 2004-11-24 2006-05-25 Frutos Anthony G Polymer-coated substrates for binding biomolecules and methods of making and using thereof
JP4585523B2 (en) 2004-12-28 2010-11-24 独立行政法人科学技術振興機構 Method for immobilizing self-organizing material or fine particles on substrate, and substrate manufactured using the method
US20060141527A1 (en) * 2004-12-29 2006-06-29 Caracci Stephen J Method for creating a reference region and a sample region on a biosensor and the resulting biosensor
US7629173B2 (en) 2004-12-29 2009-12-08 Corning Incorporated Optical reader system and method for monitoring and correcting lateral and angular misalignments of label independent biosensors
US7604984B2 (en) * 2004-12-29 2009-10-20 Corning Incorporated Spatially scanned optical reader system and method for using same
DE602006009980D1 (en) 2005-02-23 2009-12-10 Fujifilm Corp biosensor
JP2006335912A (en) * 2005-06-03 2006-12-14 Fujifilm Holdings Corp Immobilizing agent for physiologically active substance
JP2006271860A (en) * 2005-03-30 2006-10-12 Japan Lifeline Co Ltd Medical device and its manufacturing method
US8465771B2 (en) * 2005-03-30 2013-06-18 The University Of Western Ontario Anisotropic hydrogels
US20060234269A1 (en) * 2005-04-18 2006-10-19 Matthew Asplund Laser Modification and Functionalization of Substrates
JP5002584B2 (en) 2005-04-26 2012-08-15 バイエル・テクノロジー・サービシズ・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Novel apparatus and method for coating a substrate for the detection of an analytical sample by an affinity assay method
US7723438B2 (en) * 2005-04-28 2010-05-25 International Business Machines Corporation Surface-decorated polymeric amphiphile porogens for the templation of nanoporous materials
US20070009568A1 (en) * 2005-06-03 2007-01-11 Bhatnagar Rajendra S Methods for immobilizing molecules on surfaces
US8475886B2 (en) 2005-08-05 2013-07-02 Corning Incorporated Methods for producing surfaces that resist non-specific protein binding and cell attachment
WO2007035527A2 (en) * 2005-09-15 2007-03-29 Duke University Non-fouling polymeric surface modification and signal amplification method for biomolecular detection
US7763423B2 (en) * 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
US20090148346A1 (en) * 2005-10-07 2009-06-11 Canon Kabushiki Kaisha Substrate with binding functional group
JP4818056B2 (en) * 2005-10-07 2011-11-16 キヤノン株式会社 Structure having a binding functional group on a substrate for binding a capture molecule for capturing a target substance
EP1943330A4 (en) * 2005-11-01 2009-12-09 Rensselaer Polytech Inst Three-dimensional cellular array chip and platform for toxicology assays
US20070117152A1 (en) * 2005-11-08 2007-05-24 Fujifilm Corporation Biosensor
US8334140B2 (en) * 2005-11-08 2012-12-18 Smart Holograms Limited Boronate complex and its use in a glucose sensor
US20070224164A1 (en) * 2005-11-14 2007-09-27 Maciej Radosz Degradable nanogel for drug delivery
US20070110786A1 (en) * 2005-11-15 2007-05-17 Boston Scientific Scimed, Inc. Medical articles having enhanced therapeutic agent binding
US20090298197A1 (en) * 2005-11-15 2009-12-03 Oxonica Materials Inc. Sers-based methods for detection of bioagents
KR100738083B1 (en) * 2005-12-20 2007-07-12 삼성전자주식회사 A substrate used in a microarray and a method for preparing the same
US7674864B2 (en) * 2005-12-23 2010-03-09 Boston Scientific Scimed, Inc. Polymeric hybrid precursors, polymeric hybrid precursor composite matrices, medical devices, and methods
US7723422B2 (en) * 2005-12-23 2010-05-25 Boston Scientific Scimed, Inc. Functionalized block copolymers
US8455088B2 (en) * 2005-12-23 2013-06-04 Boston Scientific Scimed, Inc. Spun nanofiber, medical devices, and methods
US7781203B2 (en) * 2005-12-29 2010-08-24 Corning Incorporated Supports for assaying analytes and methods of making and using thereof
US8834912B2 (en) * 2005-12-30 2014-09-16 Boston Scientific Scimed, Inc. Medical devices having multiple charged layers
EP1808187A1 (en) * 2006-01-11 2007-07-18 Straumann Holding AG Cell selective implant surface with controlled release of bioactive agents
JP2009524834A (en) * 2006-01-27 2009-07-02 オクソニカ・インコーポレーテッド Lateral flow immunoassay using an encapsulated detection format
US8835513B2 (en) 2006-02-28 2014-09-16 Covidien Lp Drug delivery devices
US8349349B2 (en) * 2006-02-28 2013-01-08 Covidien Lp Tissue adhesives and sealants and method for their use
US7858079B2 (en) * 2006-02-28 2010-12-28 Tyco Healthcare Group Lp Tissue adhesives and sealants and method for their use
WO2007108205A1 (en) * 2006-03-17 2007-09-27 Sanyo Chemical Industries, Ltd. Cell culture substrate
US20100111917A1 (en) * 2006-03-21 2010-05-06 Ying Jackie Y Cell-adhesive polyelectrolyte material for use as membrane and coating
US8545865B2 (en) * 2006-03-24 2013-10-01 Boston Scientific Scimed, Inc. Medical devices having polymer brushes
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
EP3088083B1 (en) 2006-03-24 2018-08-01 Handylab, Inc. Method of performing pcr with a mult-ilane cartridge
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US20080050747A1 (en) * 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
US8975216B2 (en) * 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
WO2007118714A1 (en) * 2006-04-19 2007-10-25 Universiteit Gent Integrated surface mode biosensor
US8330951B2 (en) * 2006-04-28 2012-12-11 Hewlett-Packard Development Company, L.P. Nano-enhanced Raman spectroscopy substrate packaging structure
US9790538B2 (en) 2013-03-07 2017-10-17 Apdn (B.V.I.) Inc. Alkaline activation for immobilization of DNA taggants
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
EP2044402B2 (en) * 2006-07-24 2016-11-30 Becton Dickinson and Company Apparatus and method for performing an assay using magnetic particles
WO2008019381A1 (en) * 2006-08-07 2008-02-14 University Of Washington Mixed charge copolymers and hydrogels
JPWO2008018355A1 (en) * 2006-08-08 2009-12-24 シャープ株式会社 Biosensor, manufacturing method thereof, and detection method using the biosensor
JP5001015B2 (en) 2006-09-28 2012-08-15 富士フイルム株式会社 Biochemical instrument having a surface with reduced non-specific adsorption
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
AU2007323594A1 (en) * 2006-11-21 2008-05-29 Applied Biosystems, Llc Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby
US8029902B2 (en) * 2006-12-11 2011-10-04 Wisconsin Alumni Research Foundation Plasma-enhanced functionalization of substrate surfaces with quaternary ammonium and quaternary phosphonium groups
CA2674632A1 (en) * 2006-12-29 2008-07-10 University Of Washington Dual-functional nonfouling surfaces and materials
JP2008185494A (en) * 2007-01-31 2008-08-14 Fujifilm Corp Physiologically active substance-immobilized substrate
WO2008106048A1 (en) * 2007-02-28 2008-09-04 Corning Incorporated Surfaces and methods for biosensor cellular assays
JP2008249502A (en) * 2007-03-30 2008-10-16 Fujifilm Corp Solid substrate immobilized with physiologically active substance
US8093039B2 (en) * 2007-04-10 2012-01-10 The Trustees Of The Stevens Institute Of Technology Surfaces differentially adhesive to eukaryotic cells and non-eukaryotic cells
US8084077B2 (en) 2007-05-25 2011-12-27 Abbott Laboratories One-step phosphorylcholine-linked polymer coating and drug loading of stent
WO2009006445A2 (en) * 2007-06-29 2009-01-08 Applied Biosystems Systems and methods for electronic detection with nanofets
CA2693654C (en) 2007-07-13 2018-02-13 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US7960336B2 (en) * 2007-08-03 2011-06-14 Pharmain Corporation Composition for long-acting peptide analogs
US20100292427A1 (en) * 2007-08-06 2010-11-18 Canon Kabushiki Kaisha Structural member and method of producing the structural member
US8563527B2 (en) * 2007-08-20 2013-10-22 Pharmain Corporation Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same
KR101050690B1 (en) 2007-09-07 2011-07-20 광주과학기술원 Multifunctional polymer membrane and its use
WO2009031804A2 (en) * 2007-09-07 2009-03-12 Gwangju Institute Of Science And Technology Multi-functional polymeric layers and its uses
WO2009039466A1 (en) 2007-09-20 2009-03-26 Vanderbilt University Free solution measurement of molecular interactions by backscattering interferometry
DE102007049013A1 (en) * 2007-10-11 2009-04-16 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Sensor with long-term stability for organic processes
US20090130746A1 (en) * 2007-10-25 2009-05-21 Canon U.S. Life Sciences, Inc. Microchannel surface coating
US20110092768A1 (en) * 2007-10-31 2011-04-21 Given Imaging Ltd. Device, system and method for in-vivo analysis
JP5345335B2 (en) * 2007-12-03 2013-11-20 国立大学法人九州大学 Novel zwitterionic multibranched resin and protein chip surface modifier
US8304203B2 (en) 2007-12-05 2012-11-06 Zyomyx, Inc. Cell assay kit and method
US8796184B2 (en) 2008-03-28 2014-08-05 Sentilus, Inc. Detection assay devices and methods of making and using the same
US8815611B2 (en) * 2008-04-10 2014-08-26 Corning Incorporated Surface for label independent detection and method thereof
US8877298B2 (en) * 2008-05-27 2014-11-04 The Hong Kong University Of Science And Technology Printing using a structure coated with ultraviolet radiation responsive material
JP2009286968A (en) * 2008-05-30 2009-12-10 Canon Inc Graft polymer-containing substrate, its production method and magnetic biosensor
US20090311540A1 (en) * 2008-06-11 2009-12-17 Yoram Cohen Highly Sensitive and Selective Nano-Structured Grafted Polymer Layers
US20110184293A1 (en) * 2008-07-10 2011-07-28 Elisha Rabinovitz Device, method and kit for in vivo detection of a biomarker
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
WO2010005444A1 (en) * 2008-07-11 2010-01-14 Handylab, Inc. Polynucleotide capture materials, and methods of using same
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US8173198B2 (en) * 2008-07-23 2012-05-08 Life Technologies Corporation Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers
US20100034704A1 (en) * 2008-08-06 2010-02-11 Honeywell International Inc. Microfluidic cartridge channel with reduced bubble formation
US8226603B2 (en) 2008-09-25 2012-07-24 Abbott Cardiovascular Systems Inc. Expandable member having a covering formed of a fibrous matrix for intraluminal drug delivery
US8076529B2 (en) 2008-09-26 2011-12-13 Abbott Cardiovascular Systems, Inc. Expandable member formed of a fibrous matrix for intraluminal drug delivery
US8500687B2 (en) 2008-09-25 2013-08-06 Abbott Cardiovascular Systems Inc. Stent delivery system having a fibrous matrix covering with improved stent retention
US8049061B2 (en) 2008-09-25 2011-11-01 Abbott Cardiovascular Systems, Inc. Expandable member formed of a fibrous matrix having hydrogel polymer for intraluminal drug delivery
US20100099203A1 (en) * 2008-10-03 2010-04-22 Molecular Sensing, Inc. Substrates with surfaces modified with PEG
DE102008053270A1 (en) 2008-10-27 2010-05-12 Medizinische Hochschule Hannover Apparatus and method for analyzing cells
US8580240B1 (en) 2008-11-19 2013-11-12 University Of Kentucky Research Foundation Compounds and methods for reducing the occurrence of post-surgical adhesions
AU2009324879A1 (en) * 2008-11-25 2010-06-17 The Regents Of The University Of California Functionalized titanium implants and related regenerative materials
EP2192410B1 (en) * 2008-11-26 2012-07-11 Corning Incorporated Nanoparticulate affininty capture for label independent detections system
EP2204650B1 (en) * 2008-12-31 2012-06-27 DWI an der RWTH Aachen e.V. Novel biosensor system based on recognition induced birefringence (RIB)
WO2010080710A2 (en) * 2009-01-12 2010-07-15 Molecular Sensing, Inc. Sample collection and measurement in a single container by back scattering interferometry
EP2409153B1 (en) * 2009-03-20 2014-05-14 Attana AB Method of detecting ligand binding making use of a mass-sensitive sensor comprising immobilised cells on its surface
WO2010117460A1 (en) * 2009-04-08 2010-10-14 Michigan Molecular Institute Surface modification of reverse osmosis membranes by hydrophilic dendritic polymers
WO2010123448A1 (en) * 2009-04-21 2010-10-28 Gyros Patent Ab Method for reducing adsorption losses on metal oxide surfaces
US20120208291A1 (en) * 2009-05-01 2012-08-16 Univeristy Of Utah Research Foundation Methods and compositions for measuring high affinity interactions with kinetic imaging of single molecule interaction (kismi)
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US20120150015A1 (en) * 2009-09-02 2012-06-14 Mayo Foundation For Medical Education And Research System and method for detecting healing adjacent to implanted medical device
US20110225809A1 (en) * 2010-03-17 2011-09-22 Alan Francis Daher Apparatus for removably attaching an item to a surface
EP2571421B1 (en) 2010-05-17 2017-08-02 Sentilus Holdco, LLC Detection devices and related methods of use
US9638632B2 (en) 2010-06-11 2017-05-02 Vanderbilt University Multiplexed interferometric detection system and method
EP2409766A1 (en) * 2010-07-23 2012-01-25 F. Hoffmann-La Roche AG Method for hydrophilising surfaces of fluid components and objects containing such components
US9562853B2 (en) 2011-02-22 2017-02-07 Vanderbilt University Nonaqueous backscattering interferometric methods
CN106148512B (en) 2011-04-15 2020-07-10 贝克顿·迪金森公司 Scanning real-time microfluidic thermocycler and method for synchronized thermocycling and scanning optical detection
EP2541250A1 (en) 2011-06-30 2013-01-02 Koninklijke Philips Electronics N.V. Molecular architecture on magnetic particles for affinity assays with low non-specific binding
US8507294B2 (en) * 2011-07-13 2013-08-13 Empire Technology Development Llc Method and system for analyte monitoring using surface plasmons with a refreshable surface
CA2841005C (en) * 2011-07-15 2019-03-12 University Of Georgia Research Foundation, Inc. Compounds, methods of making, and methods of use
WO2013013220A2 (en) 2011-07-20 2013-01-24 University Of Washington Through Its Center For Commercialization Photonic blood typing
DK2761305T3 (en) 2011-09-30 2017-11-20 Becton Dickinson Co United Reagent Strip
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
US9518986B2 (en) 2011-11-02 2016-12-13 University Of Cape Town Method of detecting and/or quantifying an analyte in a biological sample
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
US10031138B2 (en) * 2012-01-20 2018-07-24 University Of Washington Through Its Center For Commercialization Hierarchical films having ultra low fouling and high recognition element loading properties
CN104204812B (en) 2012-02-03 2018-01-05 贝克顿·迪金森公司 The external file that compatibility determines between distributing and test for molecule diagnostic test
US9572580B2 (en) 2012-03-16 2017-02-21 Covidien Lp Closure tape dispenser
US9161756B2 (en) 2012-03-16 2015-10-20 Covidien Lp Closure tape dispenser
JP5844195B2 (en) * 2012-03-29 2016-01-13 三洋化成工業株式会社 Magnetic silica particles, immunoassay using the same, and reagent for immunoassay
US20140102899A1 (en) * 2012-04-09 2014-04-17 Applied Dna Sciences, Inc. Plasma treatment for dna binding
US9273949B2 (en) 2012-05-11 2016-03-01 Vanderbilt University Backscattering interferometric methods
EP3327090A1 (en) * 2012-07-18 2018-05-30 President and Fellows of Harvard College Modification of surfaces for simulataneous repellency and targeted binding of desired moieties
WO2014022365A1 (en) * 2012-07-31 2014-02-06 The Trustees Of Columbia University In The City Of New York Cyclopropenium polymers and methods for making the same
US9758606B2 (en) 2012-07-31 2017-09-12 The Trustees Of Columbia University In The City Of New York Cyclopropenium polymers and methods for making the same
US9297032B2 (en) 2012-10-10 2016-03-29 Apdn (B.V.I.) Inc. Use of perturbants to facilitate incorporation and recovery of taggants from polymerized coatings
US9266370B2 (en) 2012-10-10 2016-02-23 Apdn (B.V.I) Inc. DNA marking of previously undistinguished items for traceability
US8994077B2 (en) * 2012-12-21 2015-03-31 International Business Machines Corporation Field effect transistor-based bio sensor
TWI535750B (en) 2013-01-14 2016-06-01 國立臺灣大學 Polymer composition on substrate and surface modification method
WO2014171597A1 (en) 2013-04-15 2014-10-23 (주)플렉센스 Method for manufacturing nanoparticle array, surface plasmon resonance-based sensor and method for analyzing using same
US10060851B2 (en) * 2013-03-05 2018-08-28 Plexense, Inc. Surface plasmon detection apparatuses and methods
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
DE102013211850A1 (en) * 2013-06-21 2014-12-24 Gilupi Gmbh Rapid test for detection of pathogen material, in particular to support the diagnosis of sepsis, and kit and device for performing a sepsis test
DE102013211837A1 (en) * 2013-06-21 2014-12-24 Gilupi Gmbh Catheter with detection device for the real-time detection of a sample material
US9366651B2 (en) 2013-07-03 2016-06-14 Matrix Sensors, Inc. Array of sensors with surface modifications
EP3058339B1 (en) 2013-10-07 2019-05-22 APDN (B.V.I.) Inc. Multimode image and spectral reader
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
US10047282B2 (en) 2014-03-18 2018-08-14 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
WO2016004244A1 (en) 2014-07-02 2016-01-07 Life Technologies Corporation Surface treatment of semiconductor sensors
US11754563B2 (en) 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
US10760182B2 (en) 2014-12-16 2020-09-01 Apdn (B.V.I.) Inc. Method and device for marking fibrous materials
WO2016118812A1 (en) 2015-01-23 2016-07-28 Vanderbilt University A robust interferometer and methods of using same
TWI585401B (en) * 2015-12-17 2017-06-01 長庚大學 Light addressable potentiometric sensing unit
US10627396B2 (en) 2016-01-29 2020-04-21 Vanderbilt University Free-solution response function interferometry
WO2017180302A1 (en) 2016-04-11 2017-10-19 Apdn (B.V.I.) Inc. Method of marking cellulosic products
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
US9999899B2 (en) 2016-11-01 2018-06-19 International Business Machines Corporation Controlled exposure of in-vivo sensors
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
WO2018222034A1 (en) * 2017-05-30 2018-12-06 Jurriaan Huskens Surface with poly-cationic polymers and functionalized using coupling chemistry, biosensor comprising the same and use thereof in bio-sensing
WO2018234564A1 (en) * 2017-06-22 2018-12-27 Paris Sciences Et Lettres - Quartier Latin Redox cleavable comb-like copolymer for controlled adhesion between cells and substrates
WO2019189330A1 (en) 2018-03-28 2019-10-03 持田製薬株式会社 Novel reactive alginic acid derivatives
US11357542B2 (en) 2019-06-21 2022-06-14 Covidien Lp Valve assembly and retainer for surgical access assembly
EP4035735A4 (en) 2019-09-25 2023-10-18 Mochida Pharmaceutical Co., Ltd. Novel crosslinked alginic acid structure
EP4028570A1 (en) 2019-10-11 2022-07-20 Quantum-Si Incorporated Surface modification in the vapor phase
JP7343924B2 (en) * 2019-11-05 2023-09-13 国立大学法人東海国立大学機構 polypeptide
WO2023205353A1 (en) * 2022-04-21 2023-10-26 Ultima Genomics, Inc. Self assembly of beads on substrates
EP4299596A1 (en) * 2022-06-30 2024-01-03 betaSENSE GmbH Molecular probe functionalized element

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4822867A (en) * 1987-02-02 1989-04-18 Semih Erhan Protein polymer grafts
US4886836A (en) * 1987-06-03 1989-12-12 Pall Corporation Activated medium with low non-specific protein adsorption
US5866322A (en) * 1988-01-29 1999-02-02 Abbott Laboratories Method for performing Rubella assay
EP0335308B1 (en) * 1988-03-31 1993-12-08 W.R. Grace & Co.-Conn. Protein non-adsorptive polyurea-urethane polymer coated devices
JP2570408B2 (en) * 1988-11-29 1997-01-08 東ソー株式会社 Carrier for immobilizing antigen, antibody or biologically active substance
US5330911A (en) * 1989-09-28 1994-07-19 Board Of Regents, The University Of Texas System Surfaces having desirable cell adhesive effects
US5459054A (en) * 1989-12-05 1995-10-17 Neocrin Company Cells encapsulated in alginate containing a high content of a- l- guluronic acid
US5462990A (en) 1990-10-15 1995-10-31 Board Of Regents, The University Of Texas System Multifunctional organic polymers
US5529914A (en) * 1990-10-15 1996-06-25 The Board Of Regents The Univeristy Of Texas System Gels for encapsulation of biological materials
US5380536A (en) * 1990-10-15 1995-01-10 The Board Of Regents, The University Of Texas System Biocompatible microcapsules
US5626863A (en) 1992-02-28 1997-05-06 Board Of Regents, The University Of Texas System Photopolymerizable biodegradable hydrogels as tissue contacting materials and controlled-release carriers
SE467308B (en) * 1990-10-22 1992-06-29 Berol Nobel Ab SOLID SURFACE COATED WITH A HYDROPHILIC SURFACE WITH COVALENTLY BONDED BIOPOLYMERS, SET TO MAKE SUCH A SURFACE AND CONJUGATED THEREOF
US6093558A (en) * 1991-07-25 2000-07-25 Edge Biosystems, Inc. Binding protein of biologically active compositions to an adhesive formulation on a substrate
US5573934A (en) 1992-04-20 1996-11-12 Board Of Regents, The University Of Texas System Gels for encapsulation of biological materials
DE4208645A1 (en) * 1992-03-18 1993-09-23 Bayer Ag OPTICAL SOLID PHASE BIOSENSOR BASED ON FLUORESCENT COLOR-MARGINED POLYIONIC LAYERS
US5578442A (en) * 1992-03-23 1996-11-26 Vivorx, Inc. Graft copolymers of polycationic species and water-soluble polymers, and use therefor
US6113946A (en) * 1992-04-03 2000-09-05 The Regents Of The University Of California Self-assembling polynucleotide delivery system comprising dendrimer polycations
ATE154526T1 (en) * 1992-04-16 1997-07-15 Merck Patent Gmbh ACTIVATED SUPPORT MATERIALS, THEIR PRODUCTION AND USE
DE69331058D1 (en) * 1992-05-29 2001-12-06 Univ California COATED TRANSPLANT AND PRODUCTION METHOD THEREFOR
US5344455A (en) * 1992-10-30 1994-09-06 Medtronic, Inc. Graft polymer articles having bioactive surfaces
US5414075A (en) * 1992-11-06 1995-05-09 Bsi Corporation Restrained multifunctional reagent for surface modification
SE509662C2 (en) * 1993-04-29 1999-02-22 Tetra Laval Holdings & Finance Packaging laminate coated with a water-insoluble chitosan compound and methods of making the packaging laminate
US5512492A (en) * 1993-05-18 1996-04-30 University Of Utah Research Foundation Waveguide immunosensor with coating chemistry providing enhanced sensitivity
EP0700514B1 (en) * 1993-05-18 2001-11-28 University of Utah Research Foundation Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
US6284503B1 (en) * 1993-08-20 2001-09-04 University Of Utah Research Foundation Composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces
US5543326A (en) * 1994-03-04 1996-08-06 Heller; Adam Biosensor including chemically modified enzymes
AU690303B2 (en) * 1994-06-28 1998-04-23 Closure Medical Corporation Ph-modified biocompatible monomer and polymer compositions
US5795790A (en) * 1994-07-20 1998-08-18 Cytotherapeutics, Inc. Method for controlling proliferation and differentiation of cells encapsulated within bioartificial organs
US5507804A (en) * 1994-11-16 1996-04-16 Alcon Laboratories, Inc. Cross-linked polyethylene oxide coatings to improve the biocompatibility of implantable medical devices
US5932462A (en) * 1995-01-10 1999-08-03 Shearwater Polymers, Inc. Multiarmed, monofunctional, polymer for coupling to molecules and surfaces
US6017577A (en) * 1995-02-01 2000-01-25 Schneider (Usa) Inc. Slippery, tenaciously adhering hydrophilic polyurethane hydrogel coatings, coated polymer substrate materials, and coated medical devices
US5629213A (en) * 1995-03-03 1997-05-13 Kornguth; Steven E. Analytical biosensor
DE19530078A1 (en) * 1995-08-16 1997-02-20 Bayer Ag Optical solid phase biosensor based on streptavidin and biotin
US5916585A (en) * 1996-06-03 1999-06-29 Gore Enterprise Holdings, Inc. Materials and method for the immobilization of bioactive species onto biodegradable polymers
NZ333346A (en) * 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
CA2202424A1 (en) * 1997-04-11 1998-10-11 Donald E. Brooks A tethered polymer macromolecule-excluding surface, its mode of synthesis and use
DE69841937D1 (en) 1997-04-21 2010-11-25 California Inst Of Techn MULTIFUNCTIONAL POLYMER COATING
US6287558B1 (en) * 1997-08-01 2001-09-11 Biohybrio Technologies Llc Devices containing cells or tissue and an agent that inhibits damage by a host cell molecule
US6121027A (en) * 1997-08-15 2000-09-19 Surmodics, Inc. Polybifunctional reagent having a polymeric backbone and photoreactive moieties and bioactive groups
US6348322B1 (en) * 1997-10-17 2002-02-19 Duke University Method of screening for specific binding interactions
US6235340B1 (en) * 1998-04-10 2001-05-22 Massachusetts Institute Of Technology Biopolymer-resistant coatings
US6303136B1 (en) * 1998-04-13 2001-10-16 Neurotech S.A. Cells or tissue attached to a non-degradable filamentous matrix encapsulated by a semi-permeable membrane
ATE275977T1 (en) * 1998-04-13 2004-10-15 Massachusetts Inst Technology COMB POLYMERS FOR REGULATING CELL SURFACE INTERACTION
US6248127B1 (en) * 1998-08-21 2001-06-19 Medtronic Ave, Inc. Thromboresistant coated medical device
US6303179B1 (en) * 1999-02-08 2001-10-16 Medtronic, Inc Method for attachment of biomolecules to surfaces through amine-functional groups

Also Published As

Publication number Publication date
US6884628B2 (en) 2005-04-26
JP2003516519A (en) 2003-05-13
CA2371011C (en) 2009-12-22
EP1190252B1 (en) 2008-12-31
WO2000065352A1 (en) 2000-11-02
US20020128234A1 (en) 2002-09-12
AU769571B2 (en) 2004-01-29
JP4647792B2 (en) 2011-03-09
AU4684600A (en) 2000-11-10
DE60041255D1 (en) 2009-02-12
EP1190252A1 (en) 2002-03-27
ATE419528T1 (en) 2009-01-15

Similar Documents

Publication Publication Date Title
CA2371011A1 (en) Polyionic coatings in analytic and sensor devices
Löfås et al. A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands
Cosnier Affinity biosensors based on electropolymerized films
Wu et al. DNA and protein microarray printing on silicon nitride waveguide surfaces
Wink et al. Self-assembled monolayers for biosensors
WO2003093785B1 (en) Biochips with surfaces coated with polysaccharide based hydrogels
US7517705B2 (en) Phosphorus-containing polymers for optical signal transducers
CA2563404C (en) Non-covalent immobilization of indicator molecules
WO1990005303A1 (en) Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems
Gao et al. Formulation, characterization, and sensing applications of transparent poly (vinyl alcohol)− polyelectrolyte Blends
US7563623B2 (en) Biosensor
Janmanee et al. Functional conducting polymers in the application of SPR biosensors
US10077379B2 (en) Silane copolymers and uses thereof
US20150024392A1 (en) Method for producing a biosensor
EP2192409B1 (en) Label independent detection biosensor composition and methods thereof
EP2617759B1 (en) Method of modifying the properties of a surface
Coq et al. Self-supporting hydrogel stamps for the microcontact printing of proteins
EP1188042B1 (en) Method and probe for identifying a polymer
Shiota et al. Quantification of amino groups on solid surfaces using cleavable fluorescent compounds
Naraprawatphong et al. Development of protein-recognition SPR devices by combination of SI-ATRP with biomolecular imprinting using protein ligands
DK0684473T3 (en) Immunoassay element with polymers with vanadium IV (V (+4)) ions
Tlili et al. Comparative study of two surface techniques of proteins imprinting in a polydopamine matrix. Application to immunoglobulin detection
Prasad et al. Zwitterionic molecularly imprinted polymer‐based solid‐phase micro‐extraction coupled with molecularly imprinted polymer sensor for ultra‐trace sensing of L‐histidine
WO2004104223A1 (en) Method for the covalent immobilisation of probe biomolecules on organic surfaces
US20070031292A1 (en) Chemical sensors featuring dual-sensing motifs

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200428